SOLVED: The absorbance for the NADH solution is 3.0, which is too high to accurately calculate concentration.Using C1V1 = C2V2, calculate a dilution that will give you an absorbance of 0.3.Using Beer's Law and the extinction coefficient, calculate the concentration of the diluted solution. (extinction coefficient = 6220 M-1cm-1, length = 1 cm)Using the concentration of the diluted solution and the dilution factor, calculate the concentration of the original undiluted solution.
SOLVED: The absorbance for the NADH solution is 3.0, which is too hi…
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